J-domain proteins: from molecular mechanisms to diseases
Third International Workshop of Cell Stress Society International (CSSI)
| Wednesday, April 2nd | |
| 18:30 | Reception at the Novotel Gdańsk Marina Hotel |
| Thursday, April 3rd | |
| 08:30 – 9:30 | Session I: Keynote lecture – chair Jaroslaw Marszalek |
| 08:30 | Welcome speech by the Rector of the University of Gdansk |
| 08:45 | Elizabeth Craig (University of Wisconsin – Madison, USA) – ~ 50 years of Hsp70s and J-domain proteins |
| 09:30 – 10:30 | Session II: JDPs in transcriptional regulation – chair Harm Kampinga |
| 09:30 | Sebastien Dementin (CNRS-BIP Aix-Marseille University, France) – Towards a novel DnaK-dependent mechanism for the regulation of bacterial gene expression? |
| 10:00 | Axel Mogk (Heidelberg University, Germany) – An array of J-domain proteins provide synergistic control of yeast Hsf1 at distinct phases of the heat shock response |
| 10:30 – 11:00 | Group photo & Coffee break |
| 11:00 – 13:00 | Session III: JDPs in Translation- chair Harm Kampinga |
| 11:00 | Sabine Rospert (University of Freiburg, Germany) – Interplay between the components of the yeast ribosome-bound chaperone triad |
| 11:30 | David Balchin (The Francis Crick Institute, London, UK) – Chaperone coordination at the translating ribosome |
| 12:00 | Bernd Bukau, Koji Ishikawa, Paraskevi Kritsiligkou, Tobias P. Dick, Guenter Kramer (Heidelberg University, Germany) – Profiling of the nascent chain interactome of the ribosome-associated complex RAC reveals role in redox regulation of yeast cells |
| 12:30 | Claes Andreasson (Stockholm University, Sweden) – Dedicated chaperone for eEF1A at the ribosome unburdens the Hsp70 system |
| 13:00 – 14:00 | Lunch |
| 14:00 – 15:30 | Session IV: JDPs in the ER & Mitochondria – 1- chair Pierre Genevaux |
| 14:00 | Jeffrey Brodsky (University of Pittsburgh, USA) – The intersection of Hsp40 and protein ubiquitination on decision-making process in the endoplasmic reticulum |
| 14:30 | Douglas Cyr (University of North Carolina, USA) – Triage of dominantly toxic membrane proteins by DNAJB12 |
| 15:00 | Ryo Ushioda and Kasai Ayano (Kyoto Sangyo University, Japan) – ERdj8 orchestrates autophagy by regulating isolation membrane elongation |
| 15:30 – 16:00 | Coffee break |
| 16:00 – 17:30 | Session V: JDPs in the ER & Mitochondria – 2 – chair Pierre Genevaux |
| 16:00 | David Ron (University of Cambridge, UK) – Regulating BiP’s responsiveness to JDP stimulation by its reversible AMPylation/deAMPylation – an update |
| 16:30 | Dejana Mokranjac (LMU Munich, Germany) – The mitochondrial protein import motor |
| 17:00 | Christian Munch (University of Frankfurt, Germany) – DNAJA1 as a guardian of mitochondrial proteostasis |
| 17:30 – 19:00 | Dinner at the Hotel |
| 19:00 – 20:30 | Session VI: JDPs in the ER & Mitochondria – 3- Pierre Genevaux |
| 19:00 | Thomas Becker (University of Bonn, Germany) – The function of J-domain proteins in mitochondrial protein biogenesis |
| 19:30 | Fabian den Brave (University of Bonn, Germany) – Role of a J-domain protein in the quality control of mitochondrial protein biogenesis |
| 20:00 | Jaroslaw Marszalek (University of Gdansk, Poland) – Molecular bases of a shift in client binding specificity revealed by phylogeny based ancestral sequence reconstruction of an Hsp70 specialized in FeS biogenesis |
| 20:30 – 21:00 | Topic Discussion -1: JDP classification and domain organization chairs: Rina Rosenzweig/Jaroslaw Marszalek |
| Friday, April 4th | |
| 08:30 – 10:30 | Session VII: JDP-JDP interactions, disaggregation and entropic pulling – 1- chair Jaroslaw Marszalek |
| 08:30 | Matthias Mayer and Veronika Lashkul (Heidelberg University, Germany) – Functional significance of the dimeric state of J-domain proteins |
| 09:00 | Chandan Sahi (Indian Institute of Science Education and Research Bhopal, India) – Dosage compensation of Caj1-induced cytotoxicity by Sis1 and Ydj1. |
| 09:30 | Pierre Goloubinoff (University of Lausanne, Switzerland) – Hsp110 is boosting HSP70-JDP’s disaggregation activity by super-entropic pulling strokes |
| 10:00 | Paolo De Los Rios (Ecole Polytechnique Federale de Lausanne, Switzerland)- Single molecule demonstration of Entropic Pulling |
| 10:30 – 11:00 | Coffee break |
| 11:00 – 13:00 | Session VIII: JDP-JDP interactions, disaggregation and entropic pulling – 2 – chair Jaroslaw Marszalek |
| 11:00 | Krzysztof Liberek (University of Gdansk, Poland) – J-domain proteins and Hsp110 NEF in protein refolding from aggregates |
| 11:30 | Anne Wentink (Leiden University, The Netherlands) – Disaggregation of α-synuclein amyloid fibres by the human HSP70 chaperone |
| 12:00 | Kate Hyun Lee (University of Toronto, Canada) – ATP-driven remodeling of biomolecular condensates by human molecular chaperones |
| 12:30 | Harm Kampinga (University Medical Center Groningen, The Netherlands) – DNAJ involvement in disposal of aggregates |
| 13:00 – 14:00 | Lunch |
| 14:00 – 16:00 | Session IX: JDP-networks – chair Janine Kirstein |
| 14:00 | Gregory Blatch (Notre Dame University, Australia) – The chaperone partnerships of Plasmodium knowlesi, a zoonotic malaria parasite of humans |
| 14:30 | Jason Gestwicki (University of California San Francisco, USA) – Protein-protein interactions with J-domain proteins |
| 15:00 | Reut Shalgi (Technion, Israel) – Probing the chaperone network complexity |
| 15:30 | Kausik Si (Stowers Institute for Medical Research, USA) – J-domain proteins as a transducer of sensory experiences |
| 16:00 – 16:30 | Coffee break |
| 16:30 – 18:00 | Session X: Specialized JDP systems – chair Janine Kirstein |
| 16:30 | Colin Hammond (University of Liverpool, UK) – DNAJC9 integrates heat shock molecular chaperones into the histone chaperone network |
| 17:00 | Janice Braun (University Calgary, Canada) – Cellular Export of DnaJC5. |
| 17:30 | Johannes Buchner (Technical University Munich, Germany) – Evolution of JDP dependence in the Hsp90 chaperone system |
| 18:00 | Free time – dinner on your own |
| Saturday, April 5th | |
| 08:30 – 10:30 | Session XI: JDPs in protein aggregation disseases – 1 – chair Paolo De Los Rios |
| 08:30 | Sara Linse (University of Lund, Sweden) – Towards a thermodynamic understanding of solubility enhancement |
| 09:00 | Pierre Genevaux (CNRS, Toulouse University, France) – Directed evolution of DNAJ towards aggregation-prone peptides |
| 09:30 | Bartlomiej Tomiczek (University of Gdansk, Poland) – Phylogeny and ancestral sequence reconstruction support multiple origins of class B JDPs, including those involved in amyloid disaggregation |
| 10:00 | Lukasz Joachimiak (University of Texas Southwestern Medical Center, Dallas, USA) – JDP-based regulation of amyloid assembly in neurodegenerative diseases |
| 10:30 – 11:00 | Coffee break |
| 11:00 – 13:00 | Session XII: JDPs in protein aggregation disseases – 2 – chair Paolo De Los Rios |
| 11:00 | Janine Kirstein (Fritz Lipmann Institute, Jena, Germany) – Regulation of the HTT folding landscape by Hsc70, DNAJB1 and Apg |
| 11:30 | Michael Cheetham (University Collage London, UK) – DNAJ proteins in ALS/FTD |
| 12:00 | Martin Duennwald (University of Western Ontario, London, Canada) – DnaJC7 chaperones ALS-associated protein misfolding and oxidative stress |
| 12:30 | Anita Manogaran (Marquette University, USA) – Exploring the role of J domain protein chaperones on the aggregation of Transthyretin using a yeast model. |
| 13:00 – 14:00 | Lunch |
| 14:00 – 15:30 | Session XIII: JDP mutants and disease – chair Jaroslaw Marszalek |
| 14:00 | Conrad Weihl (Washington University, USA). – Pathogenic mechanisms of DNAJB proteins in myopathy and muscular dystrophy |
| 14:30 | Rina Rosenzweig (Weizmann Institute of Science, Israel) – Molecular mechanisms of DNAJC12: from structure to disease |
| 15:00 | Paul Chapple (Queen Mary University of London, UK) – DNAJ proteins in cerebellar ataxias |
| 15:30 – 16:00 | Coffee break |
| 16:00 – 17:00 | Session XIV: Conformational landscapes of JDPs – chair Jaroslaw Marszalek |
| 16:00 | Alessandro Barducci (CNRS, Montpellier University, France) – Unraveling JDP conformational landscapes: insights from molecular simulations |
| 16:30 | Yajun Jiang (Nanjing University, China) – Conformational landscape of G/F-rich region of Hsp40s |
| 17:00 – 18:00 | Topic discussion-2: JDPs as druggable targets – targeting JDP-Hsp70 and/or JDP-client interactions – chair Harm Kampinga/Paolo De Los Rios |
| 18:30 – 20:30 | Gala Dinner |
| Sunday, April 6th | |
| 08:30 | Departure |